Job Description
Media Preparation & Sterilization
* Prepare tissue culture media according to SOPs and crop-specific protocols.
* Accurately weigh and dispense macro nutrients, micro nutrients, vitamins, sugars, agar, gelling agents, and plant growth regulators.
* Monitor pH adjustment, media consistency, and gel strength.
* Operate autoclaves, media dispensers, water purification systems, and laboratory equipment.
* Ensure complete dissolution of chemicals and proper heating procedures before sterilization.
* Maintain sterility throughout the media preparation process.
Media Optimization & Troubleshooting
* Understand the effect of nutrients, hormones, and gelling agents on culture performance.
* Assist in optimizing media for multiplication, rooting, elongation, and hardening stages.
* Identify causes of poor culture growth, contamination, vitrification, browning, weak rooting, or low multiplication rates.
* Recommend adjustments in hormone concentrations, media composition, pH, agar concentration, or sterilization procedures.
* Support protocol development for banana, bamboo, blueberry, ornamentals, and other crops.
Quality Control
* Monitor media batches for consistency and quality.
* Maintain batch records and traceability of raw materials.
* Ensure adherence to laboratory SOPs and quality standards.
* Conduct periodic checks on stock solutions and chemical inventory.
Documentation
* Maintain media preparation logs and production records.
* Record observations and corrective actions.
* Support laboratory audits and compliance requirements.